TGF Beta 1 Antibody – Controlling Many Cellular Functions

TGF beta 1 antibody – Transforming Growth Factor (TGF) beta 1, also known as anti-Camurati Engelmann disease antibody, is a polypeptide member of the Transforming growth factor beta super family of cytokines. Transforming Growth Factor 1 is a secreted protein that performs many cellular functions such as controlling cell growth, proliferation, differentiation and apoptosis as well as a key role in wound healing and healthy cartilage maintenance.

The Transforming Growth Factor β 1 protein is found throughout the body and plays a role in development before birth, the formation of blood vessels, the regulation of muscle tissue and body fat development, wound healing, and immune system function. In addition, it interacts with several types of leukocytes such as T-cells, B-cells, macrophages and monocytes and plays a key role in the control of the immune system; most of the cells this cytokine regulates also secrete it. Accordingly, a lot of cells have TGFB receptors, and the protein positively and negatively regulates many other growth factors. T.G.F. β 1 is particularly abundant in tissues that make up the skeleton in addition to the extracellular matrix, highly expressed in bone and abundantly expressed in articular cartilage and chondrocytes.

Some T.G.F. β 1 gene mutations are acquired during a person’s lifetime and are present only in certain cells. These uninherited somatic mutations in TGF beta 1 gene cause alterations in the expression of the TGF β 1 protein and are associated with certain types of cancers as well as certain bone disorders. The altered protein expression may enhance several cancer related events such as proliferation, cell motility, and the development of new blood vessels that nourish a growing tumor. The TGF beta 1 protein is over expressed in certain types of prostate cancers. Altered Transforming Growth Factor beta 1 expression has also been found in breast, colon, lung, and bladder cancers. Additionally, research has shown that the TGF beta 1 antibody can operate as a biomarker and an analeptic target for cardiovascular disease, also known as heart disease. This means, using the TGF beta 1 antibody can help individuals that suffer from heart disease to determine the severity of their condition.

Aberrant Expression is also implicated in osteoarthritis, a degenerative joint disease leading to joint pain, tenderness, locking and sometimes joint effusion (increased amount of intra-articular fluid). This form of arthritis is the most common and the leading cause of chronic disability in the United States, and is affecting millions in both the United Kingdom and the United States.

Defects in T.G.F. β 1 are the cause of Camurati-Engelmann disease (CE); also known as progressive diaphyseal dysplasia 1. CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.

The host of TGF beta 1 antibody is a rabbit, and is for research purposes only. The tested applications are WB (western blot), IHC-P (immunohistochemistry), and P-Elisa.

BCL2 Antibody – Inducing Apoptosis

Bcl2 antibody – The Bcl2 gene plays an important role in a large assortment of cellular activities, in particular the regulation of cell death. This is facilitated by including or repressing apoptosis based on environmental stimuli.

Bcl2 plays an important role in the regulation of cell death, either inducing or repressing apoptosis based on environmental effects. Consequently, because of the apoptotic regulation, Bcl2 plays an important role in cancer research, cancer diagnostics and regulation of immune cells. BCL2 gene damage has been identified as a cause of a number of cancers, including melanoma, breast cancer, prostate cancer, lung cancer and certain types of leukemia.

A damaged BCL2 gene is also the cause of resistance to a number of cancer treatments. Therefore, Bcl2 antibody can be used in immunohistochemistry to distinguish cells that contain the target Bcl2 antigen. The antibodies usually react inside healthy tissues with B cells in the mantle zone, in addition to some T cells. In cancer tissues there is a substantial increase in the number of positive cells in follicular lymphoma and several other forms of cancer. On occasions, the presence or absence of Bcl2 staining in biopsies could perhaps be vital for the patient’s prognosis or the probability of a relapse.

The primary function of Bcl2 is to induce apoptosis; it does so by inducing the release of cytochrome c to begin the apoptotic cascade. Therefore, malfunctions in whichever of the Bcl2 gene or in the apoptotic cascade may possibly lead to damaged cells by missing the signal to shut down. Cancer possibly occurs as the result of an imbalance between cell death and cell growth. Anti-apoptotic protein expression and under expression of pro-apoptotic genes can, and often do, result in the lack of cell death that is characteristic of cancer. Apoptosis is very important in immune system regulation, destroying immune cells that recognize self-antigen, possibly aiding in the prevention of harmful autoimmune diseases such as Lupus and Type 1 diabetes.

The Bcl2 antibody can be used in IHC-P (immunohistochemistry) to distinguish cells that contain the target Bcl2 antigen. The antibodies usually react inside healthy tissues with B cells in the mantle zone, in addition to some T cells. Conversely, in cancer tissues there is a substantial increase in the number of positive cells in follicular lymphoma and several other forms of cancer. On occasions, the presence or absence of Bcl2 staining in biopsies could perhaps be vital for the patient’s prognosis or the probability of a relapse.

In a trial experiment the expression of Bcl2 antibody in skeletal muscles could potentially play a role on surviving muscle fibers. As the control muscles was generally positive in the nuclear membrane and cytoplasm in type 2B fibers. This experiment was conducted by using 178 biopsied human pathologic muscles and 10 control muscles by the ABC process.

The antibody can be tested on a range of applications, for instance WB (western blot), IHC-P (immunohistochemistry), and P-ELISA. This is used to test the antibody on a large assortment of model species such as mouse, rat, cow, dog, chicken, pig, and Human.

How Can I Get Healthy?

1. Pack as much nutritional punch with the foods you eat

Eat super foods as much as possible on a weekly basis. Examples of super foods are broccoli, spinach, blueberries, wild salmon, natural oats, green tea, walnuts, beans and legumes, pumpkin and squash, sweet potatoes, dark leafy vegetables, avocados, and coconuts.

2. Eliminate or reduce empty calories

The biggest culprit is sodas. Eliminate them from your daily living. Even the diet soft drinks which are worse for you than even the regular sodas. Other empty calories include candy, chips, white bread, fried foods, and most processed packaged foods.

3. Reduce Sugar

This is related to empty calories as most foods devoid of nutrition are high in sugars. Be constantly aware of how much sugar you consume everyday and take preventative measures to reduce the amount of sugar your body has to process.

4. Avoid packaged foods

If you do tend to buy some, make sure you read the ingredients in the back. Packaged foods tend to have preservatives, more salt content, higher fat, and less nutrients.

5. Eat healthy fats

Examples include uncooked extra-virgin olive oil, avocados, nuts and seeds, and coconuts.

6. Cook and eat at home

Eating out is almost never as healthy as eating at home. Fast food restaurants followed by commercial chains serve food like products that taste good but are completely devoid of nutrition. They are fatty, salty, high in hydrogenated oils, and made to purely ignite your taste buds so you can come back for more. I am not advocating fried foods but even home cooked fried chicken is better than one from KFC. If you do eat out, choose your restaurants carefully.There will be restaurants in your area that pride in their ingredients and some chefs are focusing on local organic produce to come up with innovative cuisine.

7. Eat less meat

OK, I’m not saying become a vegetarian if that’s not your thing. I’m saying simply reduce. In most American diets, meat is the main dish, reverse that make meat your side dish with a balance of more veggies and some wholesome grains. If you are a meat eater, limit your “four Legged” meat to once a week. Eliminate factory commercial animal meat products. Start buying from local small farmers who raise organic pastured animals that are not only free of hormones and antibodies, they are more nutritious. If that is not possible, most grocery stores now carry meats without hormones and antibodies.

8. Absolutely no hydrogenated fats and high fructose corn syrup (HFCS)

As you begin to read the ingredients in packaging, you will realize how many foods actually contain these two ingredients. Especially, many of the cereals have HFCS. No excuses, just make a pledge, never to eat anything with those two ingredients.Another reason to buy organic is that organic cereals and other products have better ingredients in them even if it is plain sugar over HFCS and tend to have less sugar content.

9. Eliminate as much chemicals as possible from your life

This includes, eating organic, using natural personal care products without SLS, fragrance, parabens, and other synthetic harmful ingredients. Ask your landscaper not to use round-up for weed killer as you drag that in your home with your feet. Find a non-toxic pesticide company to treat your home. I choose to live with a few spiders that are a problem in our area instead to resorting to harmful pesticide chemicals that worsen the indoor air quality of my home. Stop using commercial harmful cleaning products at home. You can literally clean your entire home with vinegar, baking soda, hydrogen peroxide and vodka (yes Vodka, as a disinfectant!). For great homemade cleaning products recipes, see my article on natural cleaning products.

10. Exercise

I know you have heard it before millions of time and this has to be on the list or else the list will not be complete. No one is saying that you have to run a marathon to get the benefits of exercise. Simply doing something active with exercise in mind 20 minutes 3-4 times a week will get you so many healthy benefits. If you’ve never exercised in your life, take baby steps (literally speaking)! Start with 10 minutes of walking, get a pedometer and hold yourself accountable to a measurable goal (2,000 steps equals 1 mile). Do something at least 4 times a week. I know there are days when you simply can’t even lift a finger but just walk or run 10 minutes if nothing else. It’s the amount of days per week that is most important. For example, it is better to run/walk 2 miles 3 times a week then walk/run 6 miles once a week.

11. Get to know your body

Start going to the doctor for annual checkups especially after the age of 35. Knowledge is power. The more you know about what’s going on with you, the more you will be proactive to take preventative measures. Know your vital numbers and make sure they are in a healthy range. If they need improvement, you can improve them with lifestyle changes rather than pharmaceutical drugs.

12. Meditate

even for 5 minutes. Don’t forget your mental attitude about life will manifest into your physical attributes and vice versa. Have faith, and you can use other mental techniques to ensure you are emotionally healthy as well. You may want to look into Emotional Freedom Techniques (EFT) as a way to help you shed your negative thoughts. EFT is something you can do on your own to help you achieve your goals and to get rid of the subconscious negative thoughts that are hindering you. And just try to be happy and avoid negative thoughts that drag you down.

13. Take care of your teeth.

Floss everyday and add 2-5 years to your life. There are a lot of studies that show that dental health is related to your overall health. Also use a toothpaste that does not contain fluoride (this is a whole new topic on why fluoride is so harmful for your health which is not in the scope of this article) and sodium laureate sulfate.

14. Take whole foods supplements

With our current diet as well as the fact that today’s soil is so depleted compared to what our parents or ancestors had, it is vital to take supplements to get the nutrients that you need to maintain a healthy diet. Your annual checkup can also reveal some deficiencies based on your lifestyle to understand what deficiencies you are dealing with. And not all supplements are created equal. There are ones that are synthetic and others that are whole foods based, you want to focus on the latter. My basic supplement list is Vit D3 (especially in the summer), probiotic, and a multi vitamin.

15. Eat more Fiber

Most of us don’t consume enough fiber. Eat steal cut oatmeal over instant oatmeal, brown rice over white rice, whole wheat over white, etc. You should have at least one Bowel movement everyday. If you regularly experience constipation and bloating, increase your fiber and add a probiotic to your supplement list. The extra good bacteria will help you balance your intestines while keep you regular with your BM.

16. Sleep

we all need it, at least 7 hours ideally 8. Sleep restores your body, your mental state and helps you cope better with the daily stresses of life. Millions of people are dealing with hormonal issues including complete adrenal fatigue. Adequate sleep truly helps you restore your body in more ways than one.

17. 80 – 20 Rule

I have applied this rule to almost everything in my life. We are not perfect, we all have weaknesses. The idea is that if you follow a healthy lifestyle 80% of the time, you will still reap the health benefits. And you give yourself a 20% margin to given in to a few life indulgences. Living healthy is a lifestyle and a habit and to know that it’s OK once in a while to eat that cake on a birthday or to have your favorite dessert is part of living while you are not giving up your healthy lifestyle. This 80-20 rule helps me deal with some of the weaknesses I battle myself. I do not eat sweet things but the chocolate lava cake at my favorite restaurant is something I won’t hold back on. The idea is that if you maintain a healthy lifestyle and occasionally give in to your weaknesses, you will still reap the benefits and you are still ahead of the game. Keep that in mind!

If this list is too overwhelming, start with one thing, and eventually incorporate the other suggestions. Even doing one of those above is a huge gift to your overall health.